Polymerase chain reaction combined with fluorescent lateral flow immunoassay based on magnetic purification for rapid detection of canine parvovirus 2

Zhuang, Linlin; Ji, Yongxin; Tian, Peilong; Wang, Kaixuan; Kou, Chengkun; Gu, Ning; Zhang, Yu

The PCR-LFIA and PCR-GE exhibit high detection efficiency, and the specificity and relevance of them were confirmed. The results obtained from this study revealed that the PCR-LFIA possesses a high specificity to CPV-2 by giving positive results for all tested isolates of CPV-2 while yielding a negative result for non-CPV strains. In the same reaction conditions, PCR-LFIA showed 100 times more sensitive than PCR-GE when 10-fold serial dilutions of standard DNA were used. The sensitivity of PCR-LFIA with standard DNA in this study was 3 × 10 1 copies/μL. The sensitivity was also higher than conventional PCR and recombinase polymerase amplification assay [41, 42] . The verification result of PCR-LFIA showed a similar sensitivity to the previously reported qPCR and real-time recombinase polymerase amplification assay [15, 43] , while the inexpensive equipment required for PCR-LFIA makes the latest valuable molecular test method, especially for resource limited setting. The PCR-LFIA saves the time required for detection, and is safe and reliable. With the LFIA reader Nanoeasy 1700, the results can be quantified and shared in real time via the internet, making it convenient for customers to communicate with doctors.