Author: Muñoz-González, Sara; Ruggli, Nicolas; Rosell, Rosa; Pérez, Lester Josué; Frías-Leuporeau, Maria Teresa; Fraile, Lorenzo; Montoya, Maria; Cordoba, Lorena; Domingo, Mariano; Ehrensperger, Felix; Summerfield, Artur; Ganges, Llilianne
Title: Postnatal Persistent Infection with Classical Swine Fever Virus and Its Immunological Implications Document date: 2015_5_4
ID: 13cii3on_20
Snippet: Moreover, to investigate the effect of IL-10 on IFN-γ production, PBMCs collected at 6 weeks p.i. from pigs 23, 25, 27 (CSFV persistently infected pigs), 32 and 33 (non-infected pigs) were cultivated in one ELISPOT assay in the presence or absence of neutralising IL-10 clone (148801, R&D System, USA) at 6 μg/10 6 cells. These cells were cultivated for duplication and were stimulated with mock, CSFV (Catalonia strain) at 0.1 MOI and PHA (10 μg/.....
Document: Moreover, to investigate the effect of IL-10 on IFN-γ production, PBMCs collected at 6 weeks p.i. from pigs 23, 25, 27 (CSFV persistently infected pigs), 32 and 33 (non-infected pigs) were cultivated in one ELISPOT assay in the presence or absence of neutralising IL-10 clone (148801, R&D System, USA) at 6 μg/10 6 cells. These cells were cultivated for duplication and were stimulated with mock, CSFV (Catalonia strain) at 0.1 MOI and PHA (10 μg/mL). These experiments were repeated twice under the same conditions.
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