Selected article for: "ELISA detect and optical density"
Author: Muñoz-González, Sara; Ruggli, Nicolas; Rosell, Rosa; Pérez, Lester Josué; Frías-Leuporeau, Maria Teresa; Fraile, Lorenzo; Montoya, Maria; Cordoba, Lorena; Domingo, Mariano; Ehrensperger, Felix; Summerfield, Artur; Ganges, Llilianne
Title: Postnatal Persistent Infection with Classical Swine Fever Virus and Its Immunological Implications
  • Document date: 2015_5_4
    • ID: 13cii3on_12
    Snippet: Anti-IFN-α monoclonal antibodies (K9 and K17) and IFN-α recombinant protein (PBL Biomedical Laboratories, Piscataway, New Jersey, USA) were used in an ELISA assay to detect IFN-α in serum samples [38] [39] [40] [41] . The cut-off value was calculated as the average of the optical density of negative controls (blank and negative serums before CSFV infection) plus three standard deviations. Cytokine concentrations in serum were determined using .....
    Document: Anti-IFN-α monoclonal antibodies (K9 and K17) and IFN-α recombinant protein (PBL Biomedical Laboratories, Piscataway, New Jersey, USA) were used in an ELISA assay to detect IFN-α in serum samples [38] [39] [40] [41] . The cut-off value was calculated as the average of the optical density of negative controls (blank and negative serums before CSFV infection) plus three standard deviations. Cytokine concentrations in serum were determined using a regression line built with the optical densities of the cytokine standards used in the test.

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