Author: Yuan, Chen; Zhang, En; Huang, Lulu; Wang, Jialu; Yang, Qian
Title: Oral administration of inactivated porcine epidemic diarrhea virus activate DCs in porcine Peyer’s patches Document date: 2018_8_16
ID: 184kmuiy_12
Snippet: DCs had the ability to stimulate lymphocyte proliferation. We examined whether DCs stimulated lymphocyte proliferation in two ways. Firstly, Different groups DCs were incubated with allogeneic lymphocyte labeled CFSE, at a rate of DCs: lymphocyte = 1: 10. Five days later, the proliferation of lymphocyte was detected by FACS.Another way, different groups DCs were incubated with allogeneic lymphocyte, five days later, the proliferation of lymphocyt.....
Document: DCs had the ability to stimulate lymphocyte proliferation. We examined whether DCs stimulated lymphocyte proliferation in two ways. Firstly, Different groups DCs were incubated with allogeneic lymphocyte labeled CFSE, at a rate of DCs: lymphocyte = 1: 10. Five days later, the proliferation of lymphocyte was detected by FACS.Another way, different groups DCs were incubated with allogeneic lymphocyte, five days later, the proliferation of lymphocyte was detected by CCK8 (Cell Counting Kit-8).
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