Oral administration of inactivated porcine epidemic diarrhea virus activate DCs in porcine Peyer’s patches

Yuan, Chen; Zhang, En; Huang, Lulu; Wang, Jialu; Yang, Qian

PEDV infected the host in the mucosal surface of the intestine. Therefore, it is important to develop oral mucosa vaccines that can elicit effective mucosal immune responses against PEDV infection. An oral vaccination is an efficacious strategy owing to induction of potent humoural and Fig. 5 The number of ileum IgA + cells increases after oral inactivated PEDV. IgA + cells were showed by immumohistochemical staining. The number of IgA + cells in per view was counted and statistical analysis was performed. Scale bar = 100 μm, n = 10, * P < 0.05, ** P < 0.01 Fig. 4 The number of ileum SWC3a + /MHC-II + DCs increases after oral inactivated PEDV. SWC3a + /MHC-II + DCs were showed by Immunofluorescence staining. The number of SWC3a + /MHC-II + DCs in per view was counted and statistical analysis was performed. Significant differences between the treated groups and the control groups are identified as. Scale bar = 100 μm, n = 10 *p < 0.05 mucosal immune responses and it offers significant advantages such as easy delivery, labor health safety in comparison to other vaccination routes such as intramuscular. Currently live attenuated PEDV vaccines are commercially used [28] . Inactivated whole-virus vaccines and live PEDV can induce significantly protective immune responses respectively. But the problem occurs when live attenuated PEDV mutates into virulent strain or the small intestine mucosal immunity is insufficient, the virus proliferates in the small intestine and can be easily spread in the piglets and cause PED. However, inactivated whole-virus vaccines can solve the secure problem and be effective as well.