Author: Shenglan Shang; Jiaqi Wu; Xiaoli Li; Xin Liu; Pan Li; Chunli Zheng; Yonghua Wang; Songqing Liu; Jiang Zheng; Hong Zhou
Title: Artesunate interacts with Vitamin D receptor to reverse mouse model of sepsis-induced immunosuppression via enhancing autophagy Document date: 2020_2_27
ID: egntml7e_43
Snippet: After total RNAs were extracted from cells and tissues using the MagMAX total RNA isolation Kit (Thermo Fisher Scientific) and transcribed into cDNAs using Prime Script (Takara, Dalian, China). Using respective primers (displayed in Supplementary Table 2), Real-time PCR was performed (7900HT Fast Real-Time PCR system or ABI 7500 Real-Time PCR system, Applied Biosystems, Darmstadt, Germany). Expression levels were normalized to β-actin. Reactions.....
Document: After total RNAs were extracted from cells and tissues using the MagMAX total RNA isolation Kit (Thermo Fisher Scientific) and transcribed into cDNAs using Prime Script (Takara, Dalian, China). Using respective primers (displayed in Supplementary Table 2), Real-time PCR was performed (7900HT Fast Real-Time PCR system or ABI 7500 Real-Time PCR system, Applied Biosystems, Darmstadt, Germany). Expression levels were normalized to β-actin. Reactions were performed in duplicate using Tli RNaseH plus and universal PCR master mix (Takara). The relative expression was calculated by the 2( -ï„ï„Ct ) method.
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