Selected article for: "lentiviral vector and sirna sequence"

Author: Shenglan Shang; Jiaqi Wu; Xiaoli Li; Xin Liu; Pan Li; Chunli Zheng; Yonghua Wang; Songqing Liu; Jiang Zheng; Hong Zhou
Title: Artesunate interacts with Vitamin D receptor to reverse mouse model of sepsis-induced immunosuppression via enhancing autophagy
  • Document date: 2020_2_27
  • ID: egntml7e_60
    Snippet: Secondly, VDR expression in RAW264.7 was modified. A VDR siRNA and a lentiviral vector harboring an RNAi sequence against VDR were transfected into RAW264.7 cells to knock down VDR expression (VDR-KD cells) ( Figure S2a) . The results showed that knock-down of VDR would lead to a loss of LPS tolerance phenotype, and addition of AS making no difference (Figure 3d) . A lentiviral vector harboring the VDR gene was transfected into RAW264.7 cells to .....
    Document: Secondly, VDR expression in RAW264.7 was modified. A VDR siRNA and a lentiviral vector harboring an RNAi sequence against VDR were transfected into RAW264.7 cells to knock down VDR expression (VDR-KD cells) ( Figure S2a) . The results showed that knock-down of VDR would lead to a loss of LPS tolerance phenotype, and addition of AS making no difference (Figure 3d) . A lentiviral vector harboring the VDR gene was transfected into RAW264.7 cells to overexpress the VDR (VDR-OE cells) ( Figure S2b ). The results showed that AS-promoting TNF-α level in LPS tolerance cells was abrogated by overexpressing VDR (Figure 3e ). These findings indicated VDR might be an essential and key molecule in LPS tolerance formation and could be further considered as an interacted candidate of AS.

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