Author: Fernandes-Matano, Larissa; Monroy-Muñoz, Irma Eloísa; Angeles-Martínez, Javier; Sarquiz-Martinez, Brenda; Palomec-Nava, Iliana Donají; Pardavé-Alejandre, Hector Daniel; Santos Coy-Arechavaleta, Andrea; Santacruz-Tinoco, Clara Esperanza; González-Ibarra, Joaquín; González-Bonilla, Cesar Raúl; Muñoz-Medina, José Esteban
Title: Prevalence of non-influenza respiratory viruses in acute respiratory infection cases in Mexico Document date: 2017_5_3
ID: 12eyo13f_15
Snippet: The SuperScript™ III Platinum 1 One-Step RT-qPCR System Kit (Invitrogen, Carlsbad, Califórnia, EUA. Catalog: 12574035) was used in a 7500 Fast Real-Time PCR System (Applied Biosystems 1 , Foster City, Califórnia, EUA) to amplify viral genetic material. Primers and probes were used for each of the following viruses: HMPV, HRSV, HPIV 1-4, betacoronavirus 1 (βCoV1), human coronavirus (HKU1, 229E, NL63) (HCoV), HMdV, RV, EV, and PBpV. The human .....
Document: The SuperScript™ III Platinum 1 One-Step RT-qPCR System Kit (Invitrogen, Carlsbad, Califórnia, EUA. Catalog: 12574035) was used in a 7500 Fast Real-Time PCR System (Applied Biosystems 1 , Foster City, Califórnia, EUA) to amplify viral genetic material. Primers and probes were used for each of the following viruses: HMPV, HRSV, HPIV 1-4, betacoronavirus 1 (βCoV1), human coronavirus (HKU1, 229E, NL63) (HCoV), HMdV, RV, EV, and PBpV. The human RNAse P (RP) gene was used as an internal control ( Table 1 ). The viruses were evaluated in uniplex reactions with the following reaction mixture: 12.5 μL of 2x Reaction Mix, 0.5 μL of each primer and probe, 1 μL of enzyme, 5.5 μL of RNAse-free water, and 5 μL of total
Search related documents:
Co phrase search for related documents- enzyme μl and human coronavirus: 1, 2, 3
- enzyme μl and probe primer: 1, 2
- enzyme μl and probe primer μl: 1, 2
- enzyme μl and reaction mixture: 1, 2, 3, 4, 5, 6
- enzyme μl and real time: 1, 2, 3, 4, 5, 6, 7, 8, 9
- enzyme μl and RNAse free water: 1
- enzyme μl and RNAse free water μl: 1
- EV RV HMdV and human coronavirus: 1
- following reaction mixture and reaction mixture: 1, 2, 3, 4
- following virus and genetic material: 1
- following virus and human coronavirus: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22
- following virus and real time: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33
- genetic material and human coronavirus: 1, 2, 3, 4, 5, 6, 7, 8
- genetic material and internal control: 1
- genetic material and probe primer: 1, 2, 3
- genetic material and real time: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21
- genetic material and viral genetic material: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74
- human coronavirus and internal control: 1, 2, 3, 4, 5
- human coronavirus and probe primer: 1, 2, 3, 4, 5
Co phrase search for related documents, hyperlinks ordered by date