Selected article for: "qpcr kit and real time"
Author: Fernandes-Matano, Larissa; Monroy-Muñoz, Irma Eloísa; Angeles-Martínez, Javier; Sarquiz-Martinez, Brenda; Palomec-Nava, Iliana Donají; Pardavé-Alejandre, Hector Daniel; Santos Coy-Arechavaleta, Andrea; Santacruz-Tinoco, Clara Esperanza; González-Ibarra, Joaquín; González-Bonilla, Cesar Raúl; Muñoz-Medina, José Esteban
Title: Prevalence of non-influenza respiratory viruses in acute respiratory infection cases in Mexico
  • Document date: 2017_5_3
    • ID: 12eyo13f_15
    Snippet: The SuperScript™ III Platinum 1 One-Step RT-qPCR System Kit (Invitrogen, Carlsbad, Califórnia, EUA. Catalog: 12574035) was used in a 7500 Fast Real-Time PCR System (Applied Biosystems 1 , Foster City, Califórnia, EUA) to amplify viral genetic material. Primers and probes were used for each of the following viruses: HMPV, HRSV, HPIV 1-4, betacoronavirus 1 (βCoV1), human coronavirus (HKU1, 229E, NL63) (HCoV), HMdV, RV, EV, and PBpV. The human .....
    Document: The SuperScript™ III Platinum 1 One-Step RT-qPCR System Kit (Invitrogen, Carlsbad, Califórnia, EUA. Catalog: 12574035) was used in a 7500 Fast Real-Time PCR System (Applied Biosystems 1 , Foster City, Califórnia, EUA) to amplify viral genetic material. Primers and probes were used for each of the following viruses: HMPV, HRSV, HPIV 1-4, betacoronavirus 1 (βCoV1), human coronavirus (HKU1, 229E, NL63) (HCoV), HMdV, RV, EV, and PBpV. The human RNAse P (RP) gene was used as an internal control ( Table 1 ). The viruses were evaluated in uniplex reactions with the following reaction mixture: 12.5 μL of 2x Reaction Mix, 0.5 μL of each primer and probe, 1 μL of enzyme, 5.5 μL of RNAse-free water, and 5 μL of total

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