Author: Sugiarto, Sarah; Spiri, Andrea M.; Riond, Barbara; Novacco, Marilisa; Oestmann, Angelina; de Miranda, Luisa H. Monteiro; Meli, Marina L.; Boretti, Felicitas S.; Hofmann-Lehmann, Regina; Willi, Barbara
Title: Passive immunization does not provide protection against experimental infection with Mycoplasma haemofelis Document date: 2016_8_5
ID: 1uw8xcxw_7
Snippet: Fifty days prior to plasma transfusion and experimental infection, the SPF status of all the cats was confirmed by testing for the absence of infection with feline hemoplasmas, feline calicivirus, feline herpesvirus-1, feline coronavirus, feline leukemia and feline immunodeficiency virus, as well as Bartonella henselae and Chlamydia felis, as recently described [19, 30] ; the absence of Mhf infection was again confirmed by real-time TaqMan ® qua.....
Document: Fifty days prior to plasma transfusion and experimental infection, the SPF status of all the cats was confirmed by testing for the absence of infection with feline hemoplasmas, feline calicivirus, feline herpesvirus-1, feline coronavirus, feline leukemia and feline immunodeficiency virus, as well as Bartonella henselae and Chlamydia felis, as recently described [19, 30] ; the absence of Mhf infection was again confirmed by real-time TaqMan ® quantitative (q)PCR [12] on day 0 prior to plasma transfusion and Mhf inoculation. All donor cats and the cat from the preexperiment were blood-typed using a commercial immunochromatography technique (Feline Lab Test A + B, Alvedia, France). The recipient cats had been bloodtyped by Liberty Research, Inc. (Waverly). A standard saline-agglutination cross-matching procedure was performed to ascertain the compatibility of transfusion [31] .
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