Author: Melnik, Lilia I; Garry, Robert F; Morris, Cindy A
Title: Peptide inhibition of human cytomegalovirus infection Document date: 2011_2_22
ID: 0p6x4lwx_25
Snippet: WWIHS is an experimentally determined algorithm that can be used to estimate the propensity of amino acid sequences to interact with lipid membrane interfaces [49] . Sequences of HCMV gB with positive WWIHS score were identified using Membrane Protein explorer (MpeX version 3.0) (http://blanco.biomol.uci.edu/mpex), a computer program based on WWIHS. HCMV gB synthetic peptides were synthesized by solid phase conventional N-α-9-fluorenylmethyloxyc.....
Document: WWIHS is an experimentally determined algorithm that can be used to estimate the propensity of amino acid sequences to interact with lipid membrane interfaces [49] . Sequences of HCMV gB with positive WWIHS score were identified using Membrane Protein explorer (MpeX version 3.0) (http://blanco.biomol.uci.edu/mpex), a computer program based on WWIHS. HCMV gB synthetic peptides were synthesized by solid phase conventional N-α-9-fluorenylmethyloxycarbonyl chemistry by Genemed Synthesis Inc. (San Francisco, CA). Peptides were purified by reverse-phase high performance liquid chromatography and confirmed by amino acid analysis and electrospray mass spectrometry. Peptide stock solutions were prepared in 10% dimethyl sulfoxide (DMSO, spectroscopy grade): 90% (v/v) H 2 O. Peptide concentrations were determined by absorbance of aromatic side chains at 280 nm (Smart-Spec ™ 3000, BioRad, Hercules, CA).
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