Author: O’Connell, Grant C.; Treadway, Madison B.; Petrone, Ashley B.; Tennant, Connie S.; Lucke-Wold, Noelle; Chantler, Paul D.; Barr, Taura L.
Title: Peripheral blood AKAP7 expression as an early marker for lymphocyte-mediated post-stroke blood brain barrier disruption Document date: 2017_4_26
ID: 1ey6ie95_18
Snippet: Blood collection and RNA extraction. Peripheral blood samples were collected via PAXgene RNA tubes (Qiagen, Valencia, CA) and stored at −80 °C until RNA extraction. RNA was extracted via PreAnalytiX PAXgene blood RNA kit (Qiagen) and automated using the QIAcube system (Qiagen). Quantity and purity of isolated RNA was determined via spectrophotometry (NanoDrop, Thermo Scientific, Waltham, MA). Quality of RNA was confirmed by chip capillary elec.....
Document: Blood collection and RNA extraction. Peripheral blood samples were collected via PAXgene RNA tubes (Qiagen, Valencia, CA) and stored at −80 °C until RNA extraction. RNA was extracted via PreAnalytiX PAXgene blood RNA kit (Qiagen) and automated using the QIAcube system (Qiagen). Quantity and purity of isolated RNA was determined via spectrophotometry (NanoDrop, Thermo Scientific, Waltham, MA). Quality of RNA was confirmed by chip capillary electrophoresis (Agilent 2100 Bioanalyzer, Agilent Technologies, Santa Clara, CA).
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