Author: O’Connell, Grant C.; Treadway, Madison B.; Petrone, Ashley B.; Tennant, Connie S.; Lucke-Wold, Noelle; Chantler, Paul D.; Barr, Taura L.
Title: Peripheral blood AKAP7 expression as an early marker for lymphocyte-mediated post-stroke blood brain barrier disruption Document date: 2017_4_26
ID: 1ey6ie95_24
Snippet: Isolation of Leukocyte Sub-populations. Blood was collected from healthy donors via K 2 EDTA vacutainer and leukocytes were isolated via immuno-magnetic negative selection (EasySep Direct, StemCell Technologies, Vancouver, CA). Neutrophils, total lymphocytes, and monocytes were isolated from 4 mLs each of EDTA-treated blood per manufacture instructions. Isolated cells were rinsed once in PBS and lysed in Qiagen buffer RLT containing beta-mercapto.....
Document: Isolation of Leukocyte Sub-populations. Blood was collected from healthy donors via K 2 EDTA vacutainer and leukocytes were isolated via immuno-magnetic negative selection (EasySep Direct, StemCell Technologies, Vancouver, CA). Neutrophils, total lymphocytes, and monocytes were isolated from 4 mLs each of EDTA-treated blood per manufacture instructions. Isolated cells were rinsed once in PBS and lysed in Qiagen buffer RLT containing beta-mercaptoethanol, flash frozen in liquid nitrogen, and stored at −80 until RNA extraction.
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