Author: Girsch, James H.; Walters, Katherine; Jackson, Wallen; Grose, Charles
Title: Progeny Varicella-Zoster Virus Capsids Exit the Nucleus but Never Undergo Secondary Envelopment during Autophagic Flux Inhibition by Bafilomycin A1 Document date: 2019_8_13
ID: 1qbklvqy_26
Snippet: Reagents. Bafilomycin A1 (B1793), as well as rabbit antibody against LC3 (L7543), was purchased from Sigma. The toxicity of BAF to cells was assessed by trypan blue exclusion. The Golgi apparatus GM130 antibody (PA1-077-A555) was obtained from Thermo Scientific. The mouse monoclonal antibody (MAb) to VZV gE (clone 3B3) was produced in our laboratory (51) . Secondary antibodies included goat anti-rabbit Alexa Fluor 488 (A11070; Life Technologies) .....
Document: Reagents. Bafilomycin A1 (B1793), as well as rabbit antibody against LC3 (L7543), was purchased from Sigma. The toxicity of BAF to cells was assessed by trypan blue exclusion. The Golgi apparatus GM130 antibody (PA1-077-A555) was obtained from Thermo Scientific. The mouse monoclonal antibody (MAb) to VZV gE (clone 3B3) was produced in our laboratory (51) . Secondary antibodies included goat anti-rabbit Alexa Fluor 488 (A11070; Life Technologies) and goat anti-mouse Alexa Fluor 546 (A11018; Life Technologies). The DNA stain was Hoechst 33342 (Invitrogen; H3570).
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