Progeny Varicella-Zoster Virus Capsids Exit the Nucleus but Never Undergo Secondary Envelopment during Autophagic Flux Inhibition by Bafilomycin A1

Girsch, James H.; Walters, Katherine; Jackson, Wallen; Grose, Charles

Archives of electron micrographs. We have retained archives of TEM negative prints in the laboratory. For the TEM results concerning the VZV infectious cycle described in a previous article from our laboratory, the published images were selected from a total of 595 micrographs taken during 12 separate experiments (21) . For a subsequent series of TEM analyses carried out as part of the research for a master's thesis, a total of 337 digital micrographs of VZV-infected cells were retained on file on a hard drive (56) .These images were captured during 10 separate experiments. The latter study included our early analyses of autophagy during VZV infection (7) . We reexamined all 932 images as part of the current study. In addition, 230 new images were collected as part of the BAF research protocol on infected monolayers.