Chemical Constituents and an Antineuroinflammatory Lignan, Savinin from the Roots of Acanthopanax henryi

Li, Xiao-Jun; Kim, Kwan-Woo; Oh, Hyuncheol; Liu, Xiang-Qian; Kim, Youn-Chul

Procedures. NMR spectra (1D and 2D) were recorded using a JEOL JNM ECP-400 spectrometer (Tokyo, Japan) (400 MHz for 1 H and 100 MHz for 13 C). HMQC and HMBC experiments were optimized for 1 CH = 140 Hz and n CH = 8 Hz, respectively. Optical rotations were recorded using a Jasco p-2000 digital polarimeter (Tokyo, Japan). HPLC (YOUNGLIN-YL9100, Younglin, Anyang, Korea) separation was performed on YMC-Pack ODS-A column (20 × 150 mm, 5 m, and 12 nm) with a flow rate of 5 mL/min and the solvents used for HPLC were analytical grade. ESI-MS data were obtained using a Q-TOF micro-LC-MS/MS instrument (Waters, Milford, MA, USA) at Korea University, Seoul, Korea. TLC was performed on Kieselgel 60 F 254 (1.05715; Merck, Darmstadt, Germany) or RP-18 F 254s (Merck) plates. Spots were visualized by spraying with 10% aqueous H 2 SO 4 solution followed by heating. Column chromatography (CC) was performed on silica gel (Kieselgel 60, 70-230 mesh and 230-400 mesh, Merck) and YMC octadecyl-functionalized silica gel (C 18 ). These procedures were based on our previous report [13] . to give a residue (303 g), which was suspended in distilled water and successively partitioned with petroleum ether (PE, b.p. 60-90 ∘ C), ethyl acetate (EtOAc), and n-butanol (BuOH), respectively.