Author: Chuck, Chi-Pang; Chow, Hak-Fun; Wan, David Chi-Cheong; Wong, Kam-Bo
Title: Profiling of Substrate Specificities of 3C-Like Proteases from Group 1, 2a, 2b, and 3 Coronaviruses Document date: 2011_11_2
ID: 0vu7bobr_17
Snippet: Our profiling analysis showed that all CoV 3CL pro prefer P5-Val and P3-Arg (Figure 1 ). To test if we can combine two favorable substitutions to create a more active substrate, we have created a doubly-substituted substrate variant 'VARLQQSGF'. The protease activities of HCoV-NL63, HCoV-OC43, SARS-CoV and IBV against the doubly-substituted sequence were 1.7060.07, 1.8760.17, 1.7060.12 and 3.2460.37, respectively ( Table 1 ). The results suggest .....
Document: Our profiling analysis showed that all CoV 3CL pro prefer P5-Val and P3-Arg (Figure 1 ). To test if we can combine two favorable substitutions to create a more active substrate, we have created a doubly-substituted substrate variant 'VARLQQSGF'. The protease activities of HCoV-NL63, HCoV-OC43, SARS-CoV and IBV against the doubly-substituted sequence were 1.7060.07, 1.8760.17, 1.7060.12 and 3.2460.37, respectively ( Table 1 ). The results suggest that the increase in activity is additive, and the sequence 'VARLQQSGF' can represent a good broad-spectrum substrate for all 3CL pro .
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