Author: Chuck, Chi-Pang; Chow, Hak-Fun; Wan, David Chi-Cheong; Wong, Kam-Bo
Title: Profiling of Substrate Specificities of 3C-Like Proteases from Group 1, 2a, 2b, and 3 Coronaviruses Document date: 2011_11_2
ID: 0vu7bobr_23
Snippet: In the FRET assay developed by us, all 3CL pro can efficiently cleave the WT sequence of 'SAVLQQSGF' with activity of 120-440 mM 21 min 21 , and the activity can be further improved by 1.7 to 3.2 fold using the substrate sequence of 'VARLQQSGF'. Because the substrate sequences can be cleaved by all 3CL pro with high efficiency, one could use the FRET assay to screen for broadspectrum inhibitors targeting 3CL pro from all groups of CoVs......
Document: In the FRET assay developed by us, all 3CL pro can efficiently cleave the WT sequence of 'SAVLQQSGF' with activity of 120-440 mM 21 min 21 , and the activity can be further improved by 1.7 to 3.2 fold using the substrate sequence of 'VARLQQSGF'. Because the substrate sequences can be cleaved by all 3CL pro with high efficiency, one could use the FRET assay to screen for broadspectrum inhibitors targeting 3CL pro from all groups of CoVs.
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