Author: Chuck, Chi-Pang; Chow, Hak-Fun; Wan, David Chi-Cheong; Wong, Kam-Bo
Title: Profiling of Substrate Specificities of 3C-Like Proteases from Group 1, 2a, 2b, and 3 Coronaviruses Document date: 2011_11_2
ID: 0vu7bobr_26
Snippet: The construction, expression and purification of the substrate library were described previously [26] . In brief, the WT substrate sequence 'TSAVLQQSGFRKM' was inserted between the cyan fluorescent protein and the yellow fluorescent protein to create the substrate protein. Saturation mutagenesis was performed at each of the P5 to P3' positions to generate a substrate library of 1968 variants......
Document: The construction, expression and purification of the substrate library were described previously [26] . In brief, the WT substrate sequence 'TSAVLQQSGFRKM' was inserted between the cyan fluorescent protein and the yellow fluorescent protein to create the substrate protein. Saturation mutagenesis was performed at each of the P5 to P3' positions to generate a substrate library of 1968 variants.
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