Progeny Varicella-Zoster Virus Capsids Exit the Nucleus but Never Undergo Secondary Envelopment during Autophagic Flux Inhibition by Bafilomycin A1

Girsch, James H.; Walters, Katherine; Jackson, Wallen; Grose, Charles

As described in Materials and Methods, we have archives of 932 transmission electron micrographs of VZV-infected monolayers without BAF treatment. Each of these transmission electron micrographs was viewed again as part of this investigation (see Fig. S2 in the supplemental material). An occasional transmission electron micrograph included an unenveloped viral particle in the cytoplasm, but we were unable to find clusters of capsids in the cytoplasm. It was uncommon to find more than a single naked viral particle along the outer nuclear membrane (ONM) of most infected cells. An occasional capsid was observed entering the inner nuclear membrane (INM). As a general comment, therefore, there appeared to be a greater number of viral particles in the perinuclear space within BAF-treated monolayers.