Author: Li, Zhao; Liu, Yong; Wei, Qingquan; Liu, Yuanjie; Liu, Wenwen; Zhang, Xuelian; Yu, Yude
Title: Picoliter Well Array Chip-Based Digital Recombinase Polymerase Amplification for Absolute Quantification of Nucleic Acids Document date: 2016_4_13
ID: 0tmd9knh_24
Snippet: Chip packaging to seal the PWA is another key technology. The picoliter droplets evaporate easily in the incubation, even when sealed with mineral oil. When the reagent concentration increases to a certain degree owing to water evaporation, the RPA reaction is not carried out in the picoliter wells, and the more demanding dRPA cannot be performed. Thus, we designed a chip chamber to seal the finished PWA chip after sample loading. The glass cover.....
Document: Chip packaging to seal the PWA is another key technology. The picoliter droplets evaporate easily in the incubation, even when sealed with mineral oil. When the reagent concentration increases to a certain degree owing to water evaporation, the RPA reaction is not carried out in the picoliter wells, and the more demanding dRPA cannot be performed. Thus, we designed a chip chamber to seal the finished PWA chip after sample loading. The glass cover-plate serves to create a vapor barrier to reduce oil and RPA reagents from evaporating during incubation [9] ; and the rubber O-ring strengthens the air tightness between the glass and the copper chamber when the screws are tightened at the four corners. Conventional dPCR can be also performed successfully on the PWA chip with the packaging device, for a thermocycling temperature and reaction time of up to 95°C and 2 h, respectively (unpublished results).
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