Selected article for: "reaction buffer and thermal cycling"
Author: Li, Zhao; Liu, Yong; Wei, Qingquan; Liu, Yuanjie; Liu, Wenwen; Zhang, Xuelian; Yu, Yude
Title: Picoliter Well Array Chip-Based Digital Recombinase Polymerase Amplification for Absolute Quantification of Nucleic Acids
  • Document date: 2016_4_13
    • ID: 0tmd9knh_26
    Snippet: As an isothermal amplification technique, RPA cannot be simply triggered using a "hot-start," which is established in conventional PCR. Instead, the RPA reaction mixture (i.e., the oligo mix, buffer, template, and enzymes) is prepared without the addition of Mg 2+ to prevent the reaction from starting. After Mg 2+ is added, the reaction proceeds, sometimes at room temperature. To disable amplification between the addition of the MgAc solution to .....
    Document: As an isothermal amplification technique, RPA cannot be simply triggered using a "hot-start," which is established in conventional PCR. Instead, the RPA reaction mixture (i.e., the oligo mix, buffer, template, and enzymes) is prepared without the addition of Mg 2+ to prevent the reaction from starting. After Mg 2+ is added, the reaction proceeds, sometimes at room temperature. To disable amplification between the addition of the MgAc solution to the RPA reaction mixture and sample loading, reagents were pre-cooled to 0°C during preparation. After the MgAc solution was added to the RPA reaction mixture, the sample loading operation was completed in less than 15 s, allowing very little time for the solution to heat or for amplification to begin. Additionally, no thermal cycling is necessary for RPA, thus enabling very easy and cheap digital amplification.

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