Author: Zhuang, Linlin; Ji, Yongxin; Tian, Peilong; Wang, Kaixuan; Kou, Chengkun; Gu, Ning; Zhang, Yu
Title: Polymerase chain reaction combined with fluorescent lateral flow immunoassay based on magnetic purification for rapid detection of canine parvovirus 2 Document date: 2019_1_17
ID: 1c5ug64m_12
Snippet: PCR products purification and LFIA detection PCR products were purified by Magbeads PCR Purification Kit (Nanoeast, Nanjing, China). According to manufacturer's instructions, 5 μL of amplified DNA was mixed with 10 μL magbeads reagent (1:2). This ratio allows for optimal selection of PCR products with fragments length greater than 100 bp. The effect of PCR products purification was simultaneously verified by 1.5% (w/v) agarose gel electrophores.....
Document: PCR products purification and LFIA detection PCR products were purified by Magbeads PCR Purification Kit (Nanoeast, Nanjing, China). According to manufacturer's instructions, 5 μL of amplified DNA was mixed with 10 μL magbeads reagent (1:2). This ratio allows for optimal selection of PCR products with fragments length greater than 100 bp. The effect of PCR products purification was simultaneously verified by 1.5% (w/v) agarose gel electrophoresis and fluorescence lateral flow immunoassay (Nanoeast, Nanjing, China).
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