Selected article for: "extraction kit and PCR amplification"
Author: Zhuang, Linlin; Ji, Yongxin; Tian, Peilong; Wang, Kaixuan; Kou, Chengkun; Gu, Ning; Zhang, Yu
Title: Polymerase chain reaction combined with fluorescent lateral flow immunoassay based on magnetic purification for rapid detection of canine parvovirus 2
  • Document date: 2019_1_17
    • ID: 1c5ug64m_17
    Snippet: The genomic DNA of CPV-2-N1 strain was extracted for PCR amplification. PCR products were purified by Magbeads PCR Purification Kit (Nanoeast, Nanjing, China). The PCR amplification products were ligated into the pUC57 vector by using Hieff Cloneâ„¢ Zero TOPO-TA Cloning Kit (Yeasen, Shanghai, China) after purified and cloned in Escherichia coli. Positive clones were identified by sequencing (GenScript, Nanjing, China). The positive clones were cu.....
    Document: The genomic DNA of CPV-2-N1 strain was extracted for PCR amplification. PCR products were purified by Magbeads PCR Purification Kit (Nanoeast, Nanjing, China). The PCR amplification products were ligated into the pUC57 vector by using Hieff Clone™ Zero TOPO-TA Cloning Kit (Yeasen, Shanghai, China) after purified and cloned in Escherichia coli. Positive clones were identified by sequencing (GenScript, Nanjing, China). The positive clones were cultured and the plasmids were extracted using Magnetic Plasmid Extraction Kit (Nanoeast, Nanjing, China), and the content of the plasmids was determined by Nano-300 Micro Spectrophotometer, then the plasmids were stored at − 20°C. The plasmid copy number was calculated following the formula [34] : copies/ μL = (6.02 × 10 23 ) × (ng/μL × 10 − 9 ) / (DNA length × 660) by using the plasmid pUC57 containing the desired fragment as a standard. Standard DNA is diluted from 3 × 10 8 to 3 × 10 0 copies/μL for PCR amplification to evaluate the detection limit of PCR-LFIA.

    Search related documents:
    Co phrase search for related documents
    • PCR amplification and standard dna: 1, 2, 3, 4, 5, 6, 7
    • PCR amplification and strain genomic dna: 1
    • PCR China Nanjing Nanoeast Purification kit and Purification kit: 1
    • PCR product and Purification kit: 1, 2, 3, 4, 5, 6, 7, 8
    • PCR product and standard dna: 1, 2, 3, 4, 5