Author: Schwarz, Megan C.; Sourisseau, Marion; Espino, Michael M.; Gray, Essanna S.; Chambers, Matthew T.; Tortorella, Domenico; Evans, Matthew J.
Title: Rescue of the 1947 Zika Virus Prototype Strain with a Cytomegalovirus Promoter-Driven cDNA Clone Document date: 2016_9_28
ID: 0i1abjfa_26
Snippet: 293T cell transfection and supernatant collection. Plasmid-based ZIKV was produced in 293T cells by transfections performed in triplicate. One day prior to transfection, 5 Ï« 10 5 cells/well were seeded in 12-well polylysine-coated plates. Cells were transfected with 0. Sequences of the primers and probe targeting ZIKV have been modified from previously published sequences (18) . Quantification of ZIKV RNA copies per milliliter of supernatant was.....
Document: 293T cell transfection and supernatant collection. Plasmid-based ZIKV was produced in 293T cells by transfections performed in triplicate. One day prior to transfection, 5 Ï« 10 5 cells/well were seeded in 12-well polylysine-coated plates. Cells were transfected with 0. Sequences of the primers and probe targeting ZIKV have been modified from previously published sequences (18) . Quantification of ZIKV RNA copies per milliliter of supernatant was performed against a standard curve of in vitro-transcribed MR766 ZIKV RNA.
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