Author: Geng, Lingling; Liu, Zunpeng; Zhang, Weiqi; Li, Wei; Wu, Zeming; Wang, Wei; Ren, Ruotong; Su, Yao; Wang, Peichang; Sun, Liang; Ju, Zhenyu; Chan, Piu; Song, Moshi; Qu, Jing; Liu, Guang-Hui
Title: Chemical screen identifies a geroprotective role of quercetin in premature aging Document date: 2018_8_1
ID: 1mrj6nb3_57
Snippet: Senescence-associated β-galactosidase (SA-β-Gal) staining was performed as described previously (Debacq-Chainiaux et al., 2009) . Briefly, hMSCs were washed in PBS and fixed at room temperature for 3 min in 2% formaldehyde and 0.2% glutaraldehyde. Fixed cells were then stained with freshly prepared staining solution for SA-β-Gal activity at 37°C overnight. Images were taken and the percentage of positive cells was calculated......
Document: Senescence-associated β-galactosidase (SA-β-Gal) staining was performed as described previously (Debacq-Chainiaux et al., 2009) . Briefly, hMSCs were washed in PBS and fixed at room temperature for 3 min in 2% formaldehyde and 0.2% glutaraldehyde. Fixed cells were then stained with freshly prepared staining solution for SA-β-Gal activity at 37°C overnight. Images were taken and the percentage of positive cells was calculated.
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