Author: Geng, Lingling; Liu, Zunpeng; Zhang, Weiqi; Li, Wei; Wu, Zeming; Wang, Wei; Ren, Ruotong; Su, Yao; Wang, Peichang; Sun, Liang; Ju, Zhenyu; Chan, Piu; Song, Moshi; Qu, Jing; Liu, Guang-Hui
Title: Chemical screen identifies a geroprotective role of quercetin in premature aging Document date: 2018_8_1
ID: 1mrj6nb3_66
Snippet: In vivo stem cell retention analysis WS hMSCs implantation was performed as previously described . In brief, 5 × 10 5 luciferase-expressing WS hMSCs were pretreated with vehicle or 100 nmol/L Que for 30 days and then implanted into the middle of the tibialis anterior muscle of immunodeficient mice. Five days after the implantation, mice were anaesthetized and injected with D-luciferin solution (Gold-Bio, luck-500). After 15 min the in vivo lucif.....
Document: In vivo stem cell retention analysis WS hMSCs implantation was performed as previously described . In brief, 5 × 10 5 luciferase-expressing WS hMSCs were pretreated with vehicle or 100 nmol/L Que for 30 days and then implanted into the middle of the tibialis anterior muscle of immunodeficient mice. Five days after the implantation, mice were anaesthetized and injected with D-luciferin solution (Gold-Bio, luck-500). After 15 min the in vivo luciferase activity of each mouse was determined by the IVIS lumina system (PerkinElmer). Luminescence intensity was normalized to luciferase intensity of hMSCs right before the implantation. All the animal experiments performed in this study were approved by the Institute of Biophysics, Chinese Academy of Sciences.
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