Selected article for: "Blue reagent and parameter sigmoidal regression"
Author: Delvecchio, Rodrigo; Higa, Luiza M.; Pezzuto, Paula; Valadão, Ana Luiza; Garcez, Patrícia P.; Monteiro, Fábio L.; Loiola, Erick C.; Dias, André A.; Silva, Fábio J. M.; Aliota, Matthew T.; Caine, Elizabeth A.; Osorio, Jorge E.; Bellio, Maria; O’Connor, David H.; Rehen, Stevens; de Aguiar, Renato Santana; Savarino, Andrea; Campanati, Loraine; Tanuri, Amilcar
Title: Chloroquine, an Endocytosis Blocking Agent, Inhibits Zika Virus Infection in Different Cell Models
  • Document date: 2016_11_29
    • ID: 01s21vh0_17
    Snippet: Vero cells, hBMEC, and NSC were incubated with medium containing chloroquine (1.56 to 200 µM) for 3 days (NSC) or 5 days (Vero and hBMEC). CellTiter Blue reagent (Promega) was added in each well, incubated for 12-16 h, and fluorescence was measured (560/590 nm). In order to calculate the 50% cytotoxicity concentration (CC50), MFI values were normalized over the untreated control. These data were plotted and Hill 4 parameter sigmoidal regression .....
    Document: Vero cells, hBMEC, and NSC were incubated with medium containing chloroquine (1.56 to 200 µM) for 3 days (NSC) or 5 days (Vero and hBMEC). CellTiter Blue reagent (Promega) was added in each well, incubated for 12-16 h, and fluorescence was measured (560/590 nm). In order to calculate the 50% cytotoxicity concentration (CC50), MFI values were normalized over the untreated control. These data were plotted and Hill 4 parameter sigmoidal regression was performed on Sigma Plot v.12.0 (Systat Software Inc.).

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