Selected article for: "PCR assay and quantitative PCR assay"
Author: Zhuang, Linlin; Ji, Yongxin; Tian, Peilong; Wang, Kaixuan; Kou, Chengkun; Gu, Ning; Zhang, Yu
Title: Polymerase chain reaction combined with fluorescent lateral flow immunoassay based on magnetic purification for rapid detection of canine parvovirus 2
  • Document date: 2019_1_17
    • ID: 1c5ug64m_33
    Snippet: In conclusion, the first quantitative fluorescent PCR-LFIA assay for CPV-2 detection was successfully established. It had the high sensitivity of 3 × 10 1 copies/μL. Cutoff value is 146. The developed PCR-LFIA is a sensitive, rapid, simple and valuable tool for quantitative detection of CPV-2 for both research and diagnostic purposes. It can also serve as a suitable molecular detection tool to facilitate timely and effective pathogenic microorg.....
    Document: In conclusion, the first quantitative fluorescent PCR-LFIA assay for CPV-2 detection was successfully established. It had the high sensitivity of 3 × 10 1 copies/μL. Cutoff value is 146. The developed PCR-LFIA is a sensitive, rapid, simple and valuable tool for quantitative detection of CPV-2 for both research and diagnostic purposes. It can also serve as a suitable molecular detection tool to facilitate timely and effective pathogenic microorganism investigations and response. Identity and divergence analysis of partial VP2 gene in CPV-2 isolates. The partial VP2 nucleotide sequences from fourteen CPV-2 isolates were sequenced and analyzed by using ClustalW method with the Lasergene MegAlign software program for percent identity and divergence. The percent sequence identity was between 99.2%~100%

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