Author: Muñoz-González, Sara; Ruggli, Nicolas; Rosell, Rosa; Pérez, Lester Josué; Frías-Leuporeau, Maria Teresa; Fraile, Lorenzo; Montoya, Maria; Cordoba, Lorena; Domingo, Mariano; Ehrensperger, Felix; Summerfield, Artur; Ganges, Llilianne
Title: Postnatal Persistent Infection with Classical Swine Fever Virus and Its Immunological Implications Document date: 2015_5_4
ID: 13cii3on_19
Snippet: To elucidate the role of IL-10 in postnatal persistence, the levels of IL-10 were firstly determined in the sera from the piglets and sows. These samples were analysed by ELISA (IL-10 Swine ELISA Kit, Life-Technologies, USA) at 7, 14, 21 and 42 days post infection (dpi). In contrast, to measure IL-10 production, 2.5 x 10 6 PBMCs/mL were cultivated for duplication in the presence or absence of neutralising IL-10 clone (148801, R&D System, USA) at .....
Document: To elucidate the role of IL-10 in postnatal persistence, the levels of IL-10 were firstly determined in the sera from the piglets and sows. These samples were analysed by ELISA (IL-10 Swine ELISA Kit, Life-Technologies, USA) at 7, 14, 21 and 42 days post infection (dpi). In contrast, to measure IL-10 production, 2.5 x 10 6 PBMCs/mL were cultivated for duplication in the presence or absence of neutralising IL-10 clone (148801, R&D System, USA) at 6 μg/10 6 cells in RPMI medium with 10% FBS. A total of 2.5x10 5 PBMCs/well were stimulated at 37°C in 96-well plates with mock, CSFV (Catalonia strain) at 0.1 MOI or PHA (10 μg/ mL). The supernatants were removed after 96 h, and the concentrations of IL-10 were determined by ELISA.
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