Author: van Zuylen, Wendy J.; Doyon, Priscilla; Clément, Jean-François; Khan, Kashif Aziz; D'Ambrosio, Lisa M.; Dô, Florence; St-Amant-Verret, Myriam; Wissanji, Tasheen; Emery, Gregory; Gingras, Anne-Claude; Meloche, Sylvain; Servant, Marc J.
Title: Proteomic Profiling of the TRAF3 Interactome Network Reveals a New Role for the ER-to-Golgi Transport Compartments in Innate Immunity Document date: 2012_7_5
ID: 1m5dbwjv_20
Snippet: Sec16A and p115 are required for TRAF3 localization to the ER-to-Golgi transport compartment and the proper recruitment to MAVS To verify this hypothesis, loss-of-function experiments were conducted using HeLa cells exposed to siRNA duplexes targeting Sec16A and p115. As previously observed for p115 and Sec16A [24, 25, 48, 49] , reducing the expression level of Sec16A or p115 led to a drastic disorganisation of the Golgi paralleled by a relocaliz.....
Document: Sec16A and p115 are required for TRAF3 localization to the ER-to-Golgi transport compartment and the proper recruitment to MAVS To verify this hypothesis, loss-of-function experiments were conducted using HeLa cells exposed to siRNA duplexes targeting Sec16A and p115. As previously observed for p115 and Sec16A [24, 25, 48, 49] , reducing the expression level of Sec16A or p115 led to a drastic disorganisation of the Golgi paralleled by a relocalization of TRAF3 as observed by the formation of small punctate structures ( Figure S4A , panels 2 and 4; Figure S4B , panel 2). However, the majority of these GM130 positive punctae do not colocalize with TRAF3 and thus appear to be different from those observed following dsRNA and dsDNA sensing (compare Figure S4A , panel 4 with Figure 4 ).
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