Author: Wang, Li; Xia, Tian; Guo, Tiantian; Ru, Yi; Jiang, Yanping; Cui, Wen; Zhou, Han; Qiao, Xinyuan; Tang, Lijie; Xu, Yigang; Li, Yijing
Title: Recombinant Lactobacillus casei Expressing Capsid Protein VP60 can Serve as Vaccine Against Rabbit Hemorrhagic Disease Virus in Rabbits Document date: 2019_11_2
ID: 1apf9w7j_17
Snippet: We used fluorescence microscopy and indirect immunofluorescence assay (IFA) to detect the expression of VP60 (VP1) and eGFP. In brief, recombinant L. casei was cultured overnight in MRS at 37 • C. Cell pellets were washed thrice with PBS, incubated with rabbit anti-VP60(VP1) hyper-immune serum(prepared in our laboratory), and then with TRITC red fluorescently labeled anti-rabbit IgG secondary antibody (diluted at 1:200) at 37 • C for 30 min. .....
Document: We used fluorescence microscopy and indirect immunofluorescence assay (IFA) to detect the expression of VP60 (VP1) and eGFP. In brief, recombinant L. casei was cultured overnight in MRS at 37 • C. Cell pellets were washed thrice with PBS, incubated with rabbit anti-VP60(VP1) hyper-immune serum(prepared in our laboratory), and then with TRITC red fluorescently labeled anti-rabbit IgG secondary antibody (diluted at 1:200) at 37 • C for 30 min. Cell pellets were then washed thrice, resuspended in 200 µL PBS, and smeared onto microscope slides. Images were obtained via laser confocal microscopy (model LSM510 META; Zeiss, Oberkochen, Germany).
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