Selected article for: "direct sequencing and dye terminator"
Author: Plyusnina, Angelina; Plyusnin, Alexander
Title: Recombinant Tula hantavirus shows reduced fitness but is able to survive in the presence of a parental virus: analysis of consecutive passages in a cell culture
  • Document date: 2005_2_22
    • ID: 04cuk2cn_17
    Snippet: A G-C A-U CCUUUAC CGGUUCA RECR855 (5'TTCTCTCCCAATTAGGTAAGCATCA3'; nt 831-855). All four primers were perfect matches to the homologous sequences; to the heterologous sequences, the forward primers have five mismatches while the reverse primers have six. Alternatively, complete S segment sequences of both variants of TULV were amplified using a single universal primer [19] and then either of the two pairs of primers was used in nested PCR. Authent.....
    Document: A G-C A-U CCUUUAC CGGUUCA RECR855 (5'TTCTCTCCCAATTAGGTAAGCATCA3'; nt 831-855). All four primers were perfect matches to the homologous sequences; to the heterologous sequences, the forward primers have five mismatches while the reverse primers have six. Alternatively, complete S segment sequences of both variants of TULV were amplified using a single universal primer [19] and then either of the two pairs of primers was used in nested PCR. Authenticity of the PCR amplicons was confirmed by direct sequencing using the ABI PRISM Dye Terminator Sequencing kit (Perkin Elmer Applied Biosystems Division).

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