Selected article for: "frameshift efficiency and stem loop"
Author: Atkins, John F.; Loughran, Gary; Bhatt, Pramod R.; Firth, Andrew E.; Baranov, Pavel V.
Title: Ribosomal frameshifting and transcriptional slippage: From genetic steganography and cryptography to adventitious use
  • Document date: 2016_9_6
    • ID: 0s8huajd_192
    Snippet: Alphaviruses continued: Though the sequence 3 of the shift site in the chikungunya-related virus, Semliki Forest virus can be drawn as a weak stem loop structure, mutagenic analysis showed no evidence for a relevant functional 3 secondary structure. Instead it was suggested that within the ribosome's mRNA entrance channel, it may act by pairing to rRNA (140) . The sequence between the shift site and the 3 pseudoknot frameshift stimulator in Midde.....
    Document: Alphaviruses continued: Though the sequence 3 of the shift site in the chikungunya-related virus, Semliki Forest virus can be drawn as a weak stem loop structure, mutagenic analysis showed no evidence for a relevant functional 3 secondary structure. Instead it was suggested that within the ribosome's mRNA entrance channel, it may act by pairing to rRNA (140) . The sequence between the shift site and the 3 pseudoknot frameshift stimulator in Middelburg virus, that in other circumstances would be called a spacer, is similar to that of Semliki Forest virus. Indeed mutating this sequence caused a drastic reduction in frameshift efficiency (140) . Further work is needed to ascertain if Middelburg virus frameshifting involves both pairing to rRNA and a pseudoknot.

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