Author: Chuck, Chi-Pang; Chow, Hak-Fun; Wan, David Chi-Cheong; Wong, Kam-Bo
Title: Profiling of Substrate Specificities of 3C-Like Proteases from Group 1, 2a, 2b, and 3 Coronaviruses Document date: 2011_11_2
ID: 0vu7bobr_3
Snippet: On the other hand, reports describing the substrate specificities of 3CL pro in group 1, 2a, and 3 are scarce. Only the activity of 3CL pro from HCoV-229E (group 1), transmissible gastroenteritis coronavirus (group 1) and mouse hepatitis virus (group 2a) against three to four of their own autocleavage sequences have been measured by Hegyi et al. [27] . Comprehensive study on substrate specificities of group 1, 2a and 3 3CL pro is lacking. Here, w.....
Document: On the other hand, reports describing the substrate specificities of 3CL pro in group 1, 2a, and 3 are scarce. Only the activity of 3CL pro from HCoV-229E (group 1), transmissible gastroenteritis coronavirus (group 1) and mouse hepatitis virus (group 2a) against three to four of their own autocleavage sequences have been measured by Hegyi et al. [27] . Comprehensive study on substrate specificities of group 1, 2a and 3 3CL pro is lacking. Here, we profiled the substrate specificities of selected 3CL pro from group 1, 2a, 2b and 3 CoVs. Activities of 3CL pro from HCoV-NL63 (group 1), HCoV-OC43 (group 2a), SARS-CoV (group 2b) and IBV (group 3) against a substrate library of 1968 variants were measured by fluorescence resonance energy transfer (FRET) assay [26] . Similarities and differences in substrate specificities among different 3CL pro are discussed.
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