Author: Zhu, Qiankun; He, Guizhen; Wang, Jie; Wang, Yukang; Chen, Wei
Title: Protective effects of fenofibrate against acute lung injury induced by intestinal ischemia/reperfusion in mice Document date: 2016_2_23
ID: 0ojbprhd_5
Snippet: Western blotting. Tissues were washed with PBS and then homogenized in lysis buffer. The extracted 50 μ g of tissue was fractionated on a Bis-Tris gel and transferred to a 0.2-μ m nitrocellulose membrane. It was blocked with 5% BSA in Tris-buffer saline with Tween-20 and incubated with a rabbit polyclonal cleaved caspase-3 (1:80; Abcam, Beijing, China) or a rabbit polyclonal Alanine Transaminase (1:1000; Abcam, Beijing, China) overnight. The me.....
Document: Western blotting. Tissues were washed with PBS and then homogenized in lysis buffer. The extracted 50 μ g of tissue was fractionated on a Bis-Tris gel and transferred to a 0.2-μ m nitrocellulose membrane. It was blocked with 5% BSA in Tris-buffer saline with Tween-20 and incubated with a rabbit polyclonal cleaved caspase-3 (1:80; Abcam, Beijing, China) or a rabbit polyclonal Alanine Transaminase (1:1000; Abcam, Beijing, China) overnight. The membranes were then incubated with horseradish peroxidase-labeled goat anti-rabbit IgG (1:3000; Abcam, Beijing, China). Bands were detected using a chemiluminescent peroxidase substrate (ECL, Amersham, Beijing, China) and exposed on a radiograph film (Kodak XBT-1, Beijing, China).
Search related documents:
Co phrase search for related documents- Bis Tris gel and Tris buffer: 1, 2, 3, 4
- Bis Tris gel and Tris buffer saline: 1
- Bis Tris gel and western blotting: 1, 2
- chemiluminescent peroxidase substrate and peroxidase substrate: 1, 2
- chemiluminescent peroxidase substrate and western blotting: 1
- lysis buffer and nitrocellulose membrane: 1, 2, 3, 4
- lysis buffer and PBS wash: 1, 2, 3, 4
- lysis buffer and rabbit polyclonal: 1, 2, 3, 4
- lysis buffer and Tris buffer: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70
- lysis buffer and Tris buffer saline: 1, 2
- lysis buffer and western blotting: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23
- nitrocellulose membrane and PBS wash: 1
- nitrocellulose membrane and peroxidase substrate: 1
- nitrocellulose membrane and rabbit polyclonal: 1
- nitrocellulose membrane and Tris buffer: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10
- nitrocellulose membrane and Tris buffer saline: 1
- nitrocellulose membrane and western blotting: 1, 2, 3, 4, 5, 6, 7
- PBS wash and Tris buffer: 1, 2, 3
- peroxidase substrate and western blotting: 1
Co phrase search for related documents, hyperlinks ordered by date