Selected article for: "anti rabbit and chemiluminescent substrate"
Author: Zhu, Qiankun; He, Guizhen; Wang, Jie; Wang, Yukang; Chen, Wei
Title: Protective effects of fenofibrate against acute lung injury induced by intestinal ischemia/reperfusion in mice
  • Document date: 2016_2_23
    • ID: 0ojbprhd_5
    Snippet: Western blotting. Tissues were washed with PBS and then homogenized in lysis buffer. The extracted 50 μ g of tissue was fractionated on a Bis-Tris gel and transferred to a 0.2-μ m nitrocellulose membrane. It was blocked with 5% BSA in Tris-buffer saline with Tween-20 and incubated with a rabbit polyclonal cleaved caspase-3 (1:80; Abcam, Beijing, China) or a rabbit polyclonal Alanine Transaminase (1:1000; Abcam, Beijing, China) overnight. The me.....
    Document: Western blotting. Tissues were washed with PBS and then homogenized in lysis buffer. The extracted 50 μ g of tissue was fractionated on a Bis-Tris gel and transferred to a 0.2-μ m nitrocellulose membrane. It was blocked with 5% BSA in Tris-buffer saline with Tween-20 and incubated with a rabbit polyclonal cleaved caspase-3 (1:80; Abcam, Beijing, China) or a rabbit polyclonal Alanine Transaminase (1:1000; Abcam, Beijing, China) overnight. The membranes were then incubated with horseradish peroxidase-labeled goat anti-rabbit IgG (1:3000; Abcam, Beijing, China). Bands were detected using a chemiluminescent peroxidase substrate (ECL, Amersham, Beijing, China) and exposed on a radiograph film (Kodak XBT-1, Beijing, China).

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