Author: Shi, Chong-Shan; Nabar, Neel R.; Huang, Ning-Na; Kehrl, John H.
Title: SARS-Coronavirus Open Reading Frame-8b triggers intracellular stress pathways and activates NLRP3 inflammasomes Document date: 2019_6_5
ID: 0fpa1f30_34
Snippet: Cell death assay 0.4% Tryptan Blue (Sigma T8154) was added to cell culture plates and incubated at room temperature for 2 min. Percentage of non-viable cells (cells that had taken up Tryptan Blue) was determined manually by light microscopy and recorded; a minimum of three fields with >50 cells per field were quantified......
Document: Cell death assay 0.4% Tryptan Blue (Sigma T8154) was added to cell culture plates and incubated at room temperature for 2 min. Percentage of non-viable cells (cells that had taken up Tryptan Blue) was determined manually by light microscopy and recorded; a minimum of three fields with >50 cells per field were quantified.
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