Selected article for: "amplified product and PCR product"
Author: Schwarz, Megan C.; Sourisseau, Marion; Espino, Michael M.; Gray, Essanna S.; Chambers, Matthew T.; Tortorella, Domenico; Evans, Matthew J.
Title: Rescue of the 1947 Zika Virus Prototype Strain with a Cytomegalovirus Promoter-Driven cDNA Clone
  • Document date: 2016_9_28
    • ID: 0i1abjfa_11
    Snippet: cells transfected with either the wild-type or the Pol(Ϫ) MR766 plasmid, as observed above ( Fig. 2A) , would require splicing of the viral RNA transcript. To directly examine this, total RNA was harvested 2 days following transfection with either plasmid, or infection with the parental MR766 virus, and RT-PCR was performed using oligonucleotides flanking the region of the intron-containing NS1 gene. As shown in Fig. 3A , a single DNA product wa.....
    Document: cells transfected with either the wild-type or the Pol(Ϫ) MR766 plasmid, as observed above ( Fig. 2A) , would require splicing of the viral RNA transcript. To directly examine this, total RNA was harvested 2 days following transfection with either plasmid, or infection with the parental MR766 virus, and RT-PCR was performed using oligonucleotides flanking the region of the intron-containing NS1 gene. As shown in Fig. 3A , a single DNA product was amplified from wild-type plasmid transfected cells that was similar in size to the parental MR766 RT-PCR product. This DNA fragment likely represented spliced NS1 RNA. However, two distinct RT-PCR products were observed from Pol(Ϫ) plasmid transfected cells, likely representing both spliced and unspliced RNA [ Fig. 3A , "Pol(Ϫ)"].

    Search related documents:
    Co phrase search for related documents
    • dna product and plasmid transfected cell: 1
    • dna product and RT PCR product: 1, 2
    • dna product and single dna product: 1, 2, 3
    • RNA transcript and RT PCR product: 1
    • RNA transcript and viral RNA transcript: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13