Author: Plyusnina, Angelina; Plyusnin, Alexander
Title: Recombinant Tula hantavirus shows reduced fitness but is able to survive in the presence of a parental virus: analysis of consecutive passages in a cell culture Document date: 2005_2_22
ID: 04cuk2cn_12
Snippet: Vero E6 cells (5 × 10 6 cells) were infected with the 1:1 mixture of recTULV and TULV02, approximately 10 4 FFU alto-gether. After 7-12 days the supernatant (~20 ml) was collected and RNA was extracted from the cells with TriPure™ isolation reagent, Boehringer Mannheim. Aliquots (2 ml) of the supernatant were used to infect fresh cells; the rest was kept at -70°C. The following nine passages were performed in the same way......
Document: Vero E6 cells (5 × 10 6 cells) were infected with the 1:1 mixture of recTULV and TULV02, approximately 10 4 FFU alto-gether. After 7-12 days the supernatant (~20 ml) was collected and RNA was extracted from the cells with TriPure™ isolation reagent, Boehringer Mannheim. Aliquots (2 ml) of the supernatant were used to infect fresh cells; the rest was kept at -70°C. The following nine passages were performed in the same way.
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