Author: Schwarz, Megan C.; Sourisseau, Marion; Espino, Michael M.; Gray, Essanna S.; Chambers, Matthew T.; Tortorella, Domenico; Evans, Matthew J.
Title: Rescue of the 1947 Zika Virus Prototype Strain with a Cytomegalovirus Promoter-Driven cDNA Clone Document date: 2016_9_28
ID: 0i1abjfa_27
Snippet: Immunoblot analysis. Immunoblot analysis was performed as previously described (19) with primary antibodies against NS3 (rabbit polyclonal antiserum 459, raised against a peptide representing amino acids 456 to 469 of the GenBank AAV34151 MR766 sequence) and actin (AC-15; Sigma-Aldrich, St. Louis, MO), horseradish peroxidase (HRP)-conjugated anti-rabbit and anti-mouse secondary antibodies (Thermo Fisher Scientific, Waltham, MA), and Immobilon che.....
Document: Immunoblot analysis. Immunoblot analysis was performed as previously described (19) with primary antibodies against NS3 (rabbit polyclonal antiserum 459, raised against a peptide representing amino acids 456 to 469 of the GenBank AAV34151 MR766 sequence) and actin (AC-15; Sigma-Aldrich, St. Louis, MO), horseradish peroxidase (HRP)-conjugated anti-rabbit and anti-mouse secondary antibodies (Thermo Fisher Scientific, Waltham, MA), and Immobilon chemiluminescent HRP detection reagent (EMD Millipore, Billerica, MA).
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