Author: Schwarz, Megan C.; Sourisseau, Marion; Espino, Michael M.; Gray, Essanna S.; Chambers, Matthew T.; Tortorella, Domenico; Evans, Matthew J.
Title: Rescue of the 1947 Zika Virus Prototype Strain with a Cytomegalovirus Promoter-Driven cDNA Clone Document date: 2016_9_28
ID: 0i1abjfa_31
Snippet: Growth curves. Vero cells (2 Ï« 10 6 ) were seeded in a 10-cm petri dish, 1 day prior to infection, in triplicate, and then infected at a multiplicity of infection (MOI) of 0.01 Vero cell TCID 50 per cell in DMEM with 2% FBS. Virus was removed 1 h later, and cells were washed twice with PBS before 6 ml of DMEM was added with 2% FBS. Supernatants were collected and filtered (0.45 m pore size) daily for the next 4 days. Supernatant infectivity was .....
Document: Growth curves. Vero cells (2 Ï« 10 6 ) were seeded in a 10-cm petri dish, 1 day prior to infection, in triplicate, and then infected at a multiplicity of infection (MOI) of 0.01 Vero cell TCID 50 per cell in DMEM with 2% FBS. Virus was removed 1 h later, and cells were washed twice with PBS before 6 ml of DMEM was added with 2% FBS. Supernatants were collected and filtered (0.45 m pore size) daily for the next 4 days. Supernatant infectivity was assayed as previously described.
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