Author: Zhao, Huabin; Ru, Binghua; Teeling, Emma C.; Faulkes, Christopher G.; Zhang, Shuyi; Rossiter, Stephen J.
Title: Rhodopsin Molecular Evolution in Mammals Inhabiting Low Light Environments Document date: 2009_12_16
ID: 02uqygfs_41
Snippet: Genomic DNA was extracted from either muscle tissue or, for bats, wing membrane biopsies, using Qiagen DNeasy kits. The rhodopsin gene includes five exons interrupted by four introns. Three primer pairs were designed from conserved regions of primates, rodents, cow and dog and used to amplify three overlapping fragments (see Table S2 , Supplementary Material online). For one taxon (Hottentot golden mole) these did not work and thus four additiona.....
Document: Genomic DNA was extracted from either muscle tissue or, for bats, wing membrane biopsies, using Qiagen DNeasy kits. The rhodopsin gene includes five exons interrupted by four introns. Three primer pairs were designed from conserved regions of primates, rodents, cow and dog and used to amplify three overlapping fragments (see Table S2 , Supplementary Material online). For one taxon (Hottentot golden mole) these did not work and thus four additional primer pairs were used to amplify exon by exon (see Table S2 , Supplementary Material online).
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