Author: Zhao, Huabin; Ru, Binghua; Teeling, Emma C.; Faulkes, Christopher G.; Zhang, Shuyi; Rossiter, Stephen J.
Title: Rhodopsin Molecular Evolution in Mammals Inhabiting Low Light Environments Document date: 2009_12_16
ID: 02uqygfs_6
Snippet: We sequenced approximately 3.3 kb of the rhodopsin gene from 22 mammal species and analysed our new data along with the published sequences of an additional 37 mammal species. All new sequences were found to have strictly conserved intronicsplice signals (GT/AG) and, based on the amplification of mRNA from seven bat species, we found no differences between genomic DNA and coding sequences. In total, we obtained 983 bp of genomic DNA for comparati.....
Document: We sequenced approximately 3.3 kb of the rhodopsin gene from 22 mammal species and analysed our new data along with the published sequences of an additional 37 mammal species. All new sequences were found to have strictly conserved intronicsplice signals (GT/AG) and, based on the amplification of mRNA from seven bat species, we found no differences between genomic DNA and coding sequences. In total, we obtained 983 bp of genomic DNA for comparative analyses, representing 94% of the coding sequence (1047 bp) and including all transmembrane (TM) helical regions, as well as extracellular domains implicated in the function of visual pigments [29] . We identified 327 amino acids and no premature stop codons were detected. With one exception, none of the new sequences contained insertions or deletions when compared to the 37 published sequences on GenBank. However, the afrotherian Hottentot golden mole showed one 3 basepair deletion that was in frame.
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