Revalidation and genetic characterization of new members of Group C (Orthobunyavirus genus, Peribunyaviridae family) isolated in the Americas

Nunes, Márcio Roberto Teixeira; de Souza, William Marciel; Acrani, Gustavo Olszanski; Cardoso, Jedson Ferreira; da Silva, Sandro Patroca; Badra, Soraya Jabur; Figueiredo, Luiz Tadeu Moraes; Vasconcelos, Pedro Fernando da Costa

The topology of the ML tree for the M segment reveals four phylogenetic groups that were well supported (bootstrap > 80%). These groups were named following previous serological classifications based on complement fixation, neutralization, and hemagglutination inhibition tests: Marituba, Caraparu, Madrid and Oriboca complexes (Fig 3B) [1, 27] . The Caraparu complex includes CARV, APEUV and BRUV, Madrid complex comprises MADV and ITYV, Marituba complex comprises MTBV, MURV, RESV, NEPV and GLV, and the Oriboca complex was composed by ORIV and ITQV [1] . Unfortunately, there are no complete sequences available to Vinces and Ossa viruses, but probably these viruses will be clustered into Caraparu complex as previously showed by serological assays [28] . Possibly, the concordance observed between the phylogeny of the M segment and the serological classification occurs because the M segment encodes the glycoproteins, which are the primary antigenic determinants recognized by the neutralization and hemagglutination inhibition tests [1, 29] .