Selected article for: "activation pathway and dimer formation"

Author: Brisse, Morgan; Ly, Hinh
Title: Comparative Structure and Function Analysis of the RIG-I-Like Receptors: RIG-I and MDA5
  • Document date: 2019_7_17
  • ID: 1enteev7_22
    Snippet: The minimum length of dsRNA found to activate RIG-I is 13 base pairs, which is equivalent to the minimum length to facilitate the formation of a 2-RIG-I/dsRNA dimer (75) . That being said, shorter (∼10 bp) 5 ′ ppp stem loop dsRNA complexes that have previously been used to obtain X-ray crystallographic structures of RIG-I interacting with dsRNA (61, 134, 135) (Figure 3C ) can also activate IFNβ signaling in cells (135, 136) and in mice (136).....
    Document: The minimum length of dsRNA found to activate RIG-I is 13 base pairs, which is equivalent to the minimum length to facilitate the formation of a 2-RIG-I/dsRNA dimer (75) . That being said, shorter (∼10 bp) 5 ′ ppp stem loop dsRNA complexes that have previously been used to obtain X-ray crystallographic structures of RIG-I interacting with dsRNA (61, 134, 135) (Figure 3C ) can also activate IFNβ signaling in cells (135, 136) and in mice (136) . Furthermore, A549 cells that were transfected with RIG-I plasmid 6 h prior to RNA transfection had a minimum dsRNA length of only 8-10 bp required for activation (75) . This indicates that RIG-I oligomerization may not be necessary for activation of the IFNβ pathway under some experimental conditions, which need to be further investigated.

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