Author: Schwarz, Megan C.; Sourisseau, Marion; Espino, Michael M.; Gray, Essanna S.; Chambers, Matthew T.; Tortorella, Domenico; Evans, Matthew J.
Title: Rescue of the 1947 Zika Virus Prototype Strain with a Cytomegalovirus Promoter-Driven cDNA Clone Document date: 2016_9_28
ID: 0i1abjfa_28
Snippet: Immunofluorescence. Cells were fixed with 4% paraformaldehyde and stained with the rabbit polyclonal antiserum 459 against ZIKV NS3 or a primary antibody targeting the flavivirus E proteins (clone D1-4G2-4-15; EMD Millipore) and a goat anti-rabbit or a goat anti-mouse secondary antibody conjugated to Alexa Fluor 568 and 488 (Thermo Fisher Scientific, Waltham, MA), respectively, using methods previously described (14) . The nucleus was visualized .....
Document: Immunofluorescence. Cells were fixed with 4% paraformaldehyde and stained with the rabbit polyclonal antiserum 459 against ZIKV NS3 or a primary antibody targeting the flavivirus E proteins (clone D1-4G2-4-15; EMD Millipore) and a goat anti-rabbit or a goat anti-mouse secondary antibody conjugated to Alexa Fluor 568 and 488 (Thermo Fisher Scientific, Waltham, MA), respectively, using methods previously described (14) . The nucleus was visualized with Hoechst stain (Thermo Fisher Scientific, Waltham, MA). Images were acquired with the Evos microscope (Thermo Fisher Scientific, Waltham, MA).
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