Author: Schwarz, Megan C.; Sourisseau, Marion; Espino, Michael M.; Gray, Essanna S.; Chambers, Matthew T.; Tortorella, Domenico; Evans, Matthew J.
Title: Rescue of the 1947 Zika Virus Prototype Strain with a Cytomegalovirus Promoter-Driven cDNA Clone Document date: 2016_9_28
ID: 0i1abjfa_29
Snippet: Flow cytometry. Cells were fixed with 4% paraformaldehyde and stained with the primary 4G2 flavivirus E antibody (EMD Millipore, Billerica, MA) and a goat anti-mouse secondary antibody conjugated to Alexa Fluor 647 (Thermo Fisher Scientific, Waltham, MA), using methods previously described (20) . Cells were analyzed using an LSRII flow cytometer (Becton, Dickinson, Franklin Lakes, NJ)......
Document: Flow cytometry. Cells were fixed with 4% paraformaldehyde and stained with the primary 4G2 flavivirus E antibody (EMD Millipore, Billerica, MA) and a goat anti-mouse secondary antibody conjugated to Alexa Fluor 647 (Thermo Fisher Scientific, Waltham, MA), using methods previously described (20) . Cells were analyzed using an LSRII flow cytometer (Becton, Dickinson, Franklin Lakes, NJ).
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