RNA disruption is associated with response to multiple classes of chemotherapy drugs in tumor cell lines

Narendrula, Rashmi; Mispel-Beyer, Kyle; Guo, Baoqing; Parissenti, Amadeo M.; Pritzker, Laura B.; Pritzker, Ken; Masilamani, Twinkle; Wang, Xiaohui; Lannér, Carita

To investigate the relationship between drug sensitivity and drug-induced RNA disruption, total RNA was isolated from docetaxel-sensitive (A2780) and docetaxel-resistant (A2780DXL) cells after treatment with 0.005 and 0.2 μM docetaxel for 48 or 72 h. Consistent with their known drug sensitivity using clonogenic assays [23] , A2780 cells exhibited a significantly higher RNA disruption index than A2780DXL cells did (Fig. 5a) [23] . Using RDI analysis we were able to confirm this response, as significantly higher RDI values were observed in the treated resistant cells when compared to the untreated resistant cells, demonstrating sensitivity of the A2780DXL cells to carboplatin and of the A2780CBN cells to docetaxel (Fig. 5c and d) . RDA consistently reflected the above differential drug sensitivities, by displaying higher RDI values and RNA disruption bands in drug-sensitive cells (Additional file 5A, B, C, D).