Selected article for: "flow cytometry and propidium iodide"

Author: Narendrula, Rashmi; Mispel-Beyer, Kyle; Guo, Baoqing; Parissenti, Amadeo M.; Pritzker, Laura B.; Pritzker, Ken; Masilamani, Twinkle; Wang, Xiaohui; Lannér, Carita
Title: RNA disruption is associated with response to multiple classes of chemotherapy drugs in tumor cell lines
  • Document date: 2016_2_24
  • ID: 0mjizsoo_18
    Snippet: To analyze the effect of docetaxel on the proportion of cells entering apoptosis, cells were stained with annexin V and propidium iodide (PI) (CytoGLO Annexin V-FTIC Apoptosis Kit, IMGENEX, San Diego, CA, USA) and the percentage of apoptotic cells (annexin V positive, PI negative) was determined by flow cytometry on a BD FACS Canto II flow cytometer (Becton-Dickinson Biosciences, Mississauga, ON, CA). The effect of docetaxel on cell cycle progres.....
    Document: To analyze the effect of docetaxel on the proportion of cells entering apoptosis, cells were stained with annexin V and propidium iodide (PI) (CytoGLO Annexin V-FTIC Apoptosis Kit, IMGENEX, San Diego, CA, USA) and the percentage of apoptotic cells (annexin V positive, PI negative) was determined by flow cytometry on a BD FACS Canto II flow cytometer (Becton-Dickinson Biosciences, Mississauga, ON, CA). The effect of docetaxel on cell cycle progression was also assessed by flow cytometry after cells were fixed and stained with PI alone as described previously [27] .

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